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For 2D SDS-PAGE, GST samples were rehydrated and focused on 7 cm pH 3 10NL IPG strips to 10 000 Vh, using a Protean IEF Cell (Bio-Rad)

Bikle DD, Vitamin D (2014) metabolism, mechanism of action, and clinical applications

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Much of that benefit is reported anecdotally and has not been confirmed in large, rigorous human trials

From a mechanistic perspective, the contraction of muscle fibers and the hemodynamic forces elicited by exercise activate a complex network of intracellular signaling pathways which collectively enhance mitochondrial biogenesis and quality control, stimulate angiogenic processes, and augment eNOS activity along with NO production (73, 74)