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As shown in Figure 2b, the levels of transcript abundance increased two to three fold in resistant plants after Hessian fly infestation when the common primer pair was used for qPCR analysis

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Peptides were separated using an Acclaim Pepmap 100 trap column (75 micron ID 2 cm from Themo Scientific) and eluted onto an Acclaim PepMap RSKC analytical column (75 micron ID x 2 cm, Thermo Scientific) with a gradient of solvent A (water and 0.1% formic acid) and solvent B (acetonitrile and 0.1% formic acid)

As with other high-coordinate structures the energy difference between these eightfold coordination geometries is small enough that packing effects can significantly influnece the observed structure

S6b, c)