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Basic residues (such as arginine) are paired with nonpolar amino acids (such as proline, alanine, valine and leucine), which have a strong inhibitory effect on tyrosinase (Zu et al., 2023)

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Common lab protocols involve reconstitution in bacteriostatic water and subcutaneous injection in animal models using calibrated syringes to achieve precise dosing

The animals were placed in the stereotaxic device (David Kopf Instruments, Tujunga, CA, USA) and holes were drilled in the frontal, parietal, and occipital regions of the skull at the following locations: 2 mm from Bregma, 2 mm lateral to the midline (ipsilateral), and 2 mm posterior to Lambda (reference electrode)

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