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The supernatant containing viral particles was harvested at 48 and 72 h after transfection, and was filtered through Millex-GP Filter Unit (0.45 m pore size, Millipore)

This was supported by the decreased levels of serine in the medium of 1919 cells compared to input media and 2012.2 cells ( p = 0.0129, p = 0.0027, respectively), as well as reduced synthesized serine and glycine into the media (Fig

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These cells produce nitric oxide, which keeps blood vessels healthy

TFR2, a driver of ferroptosis, promotes cellular iron transport