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After three washes with TBST, membranes were detected by Enhanced Chemiluminescence with images captured by the ChemiDoc MP imaging system (Bio-rad, California, USA)

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Supplementation with a combination of fish oil containing FAs and vitamins and minerals to males (W8-16) had no effect on POS-induced depressive-like behavior in the FST at W10-16 [122], while in females supplemented with choline, betaine, folic acid and vitamin B12 (P60-P186) the POS-induced depressive-like behavior was ameliorated in the FST at P165-186 [123]