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Clin Immunol (2013) 149(3):55665

Following incubation, the resin was washed thrice on a midi batch purification column (Generon, Maidenhead, UK) using wash buffer (20 mM Tris-HCl, pH 7.8, 0.5 M NaCl, 5 M urea, 10-50 mM imidazole) before elution in buffer containing 20 mM Tris-HCl, pH 7.8, 0.5 M NaCl, 300 mM imidazole and 5 M urea

Our findings support prior clinical observations that melatonin signaling is dysregulated in asthma and highlight the importance of temporal and cell-intrinsic context in determining its immunomodulatory effects

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These restorative effects combine cellular repair with noticeable improvements in texture and tone