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Check whether the SSA concentration is 1% or less at the time of measurement
Primary ESCs were treated with or without the cyst fluid (1:1 diluted with complete medium) or dimethyl sulphoxide (DMSO) or 30 M erastin for 12 h and then were washed thrice with PBS and fixed with 2.5% glutaraldehyde at 4 C overnight
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In experimental heart transplantation, NDP-alpha-MSH, a synthetic analogue of alpha-melanocyte-stimulating hormone (-MSH), demonstrated protective activity by influencing various molecular pathways associated with heart function preservation
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