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1g of total RNA for each sample was used for poly(A) RNA selection using magnetic beads coated with poly-dT, followed by thermal fragmentation

The key question is whether the model requires prolonged GH-axis stimulation or shorter-duration GHRH signaling

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While these results support the potential of this platform for targeted antioxidant therapy, further investigations are required to optimize its formulation, evaluate long-term safety, and assess translational feasibility