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Optimise general joint health through evidence-based lifestyle measures: Maintain a healthy weight to reduce mechanical stress on weight-bearing joints Engage in regular low-impact exercise such as swimming, cycling, or walking to maintain joint mobility and strengthen supporting muscles Apply heat or cold therapy as appropriateheat for stiffness, cold for acute inflammation Follow a balanced diet with plenty of fruits, vegetables, and whole grains

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Additionally, ELISA was performed to quantify secreted IL-6 and TGF- in co-culture media [2]