Lentiviral transduction Lentiviral particles were produced by transfecting Lenti-X 293T cell line as previously described [65]
For elution of the target protein, the interaction to the ligand is resolved by changing the buffer conditions, for example, the pH value, or by adding a specific competitor that supplants the target protein from the ligand resulting in dissociation of the target, while the ligand remains immobilized on the stationary phase
Strosberg J, El-Haddad G, Wolin E, Hendifar A, Yao J, Chasen B
Mechanical approaches Microneedle arrays were created to alleviate the drawbacks of hypodermic needles and to administer drugs that could not diffuse through the skin using passive or externally assisted diffusion [263, 264]
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