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Continuous administration does not seem to provide additional benefit and may actually diminish the compound's effectiveness over time

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Depression, the Val66Met polymorphism, age and gender influence the serum BDNF level

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Week 1: Dose: 50mcg Frequency: Once, on a night when you can sleep in the next morning Timing: 2-3 hours before intended sleep Purpose: Assess basic tolerance and response Week 2: Dose: 75-100mcg Frequency: 2-3 times during the week Timing: 1-2 hours before bed Purpose: Establish baseline effective dose During this assessment phase, track sleep quality subjectively and, if possible, with a wearable device that measures sleep stages