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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Research into Extracellular Matrix Dynamics Epithalon has been explored for its possible impact on extracellular matrix (ECM) proteins, such as MMP2, which are associated with tissue remodeling

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Knopman DS, Amieva H, Petersen RC, Chtelat G, Holtzman DM, Hyman BT, Nixon RA, Jones DT (2021) Alzheimer disease