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Although the mechanisms of acquired resistance in situ are still poorly understood, studies in vitro have shown that cells selected for resistance to one drug often exhibit cross-resistance to other seemingly unrelated agents, suggesting a somewhat generalised mechanism of resistance

Addition of MBB (30 l of stock solution (1mM)) started the reaction and was followed as above

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3A), hence supporting our hypothesis that RSL3-induced PTC cell death is indeed a ferroptosis pathway-dependent phenomenon