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For this study, a nested casecontrol design was employed in which patients were selected based on their overweight/obesity status and control subjects were age- and sex-matched subjects, both recruited at a ratio of 1:1.6

10.1016/j.lfs.2004.04.038 30 ChauhanA.GuF.EssaM

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Uchida Y, Yagi Y, Takao M, Tano M, Umetsu M, Hirano S, et al

Following this, 20 L cAMP-Glo Lysis Buffer was added to each well, and the plate was incubated for 15 min, prior to the addition of PKA and cAMP-Glo Reaction Buffer, and the procedure above followed