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Potential renal stress markers reported in rodent studies at higher doses

Sequence analysis can be made by polymerase chain reaction (PCR) and sequence analysis of the -glutamylcysteine synthetase genes ( GLCLC , GLCLR ) [1]

Intravenous (IV) nutrition is much more than intravenous vitamins, minerals, amino acids and other nutrients

When to increase dose Consider increasing if: Minimal response after 4-6 weeks at current dose No side effects at current dose Current dose below the higher end of recommended range Goals not being met with conservative approach Increase gradually, typically by 25-50% at a time

Plates were placed in a motorized stage microscope (EVOS Auto FL 2, Thermo Fisher Scientific, Waltham, MA) equipped with an on-stage incubator and maintained at 5% CO 2 and 37C