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However, further efforts are needed to standardize in vitro protocols for evaluating Epitalons biological activity and to clarify its precise molecular mechanisms, which remain only partially understood

The goal is gentle optimization, not overwhelming your system with multiple interventions

At the same time, it preserved the homeostasis of CD8+ splenocytes, maintaining their cytotoxic function without provoking an excessive inflammatory response [50], suggesting Epitalons role in recalibrating, rather than overactivating, immune function

Cells were solubilized by 1% NP-40 in PBS, and competent BL21 bacteria were added

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