Rllin HB, Theodorou P, Kilroe-Smith TA (1991) The effect of exposure to aluminium on concentrations of essential metals in serum of foundry workers
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Think of them as part of the support crew that makes the real work easier
A smaller quantity may lead to frequent reorders, while a larger quantity may not be suitable if the plan is still being adjusted
As for any metabolite assay, it is advisable to check extraction efficiency, for example, with recovery experiments using known amounts of GSH or GSSG added at the stage of extraction in the presence or absence of the tissue under examination