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If a patient has uncontrolled hypothyroidism, it is crucial to consider the potential risks of using VLCKD, including dyslipidemia, osteoporosis, depression, gastrointestinal effects, and cardiomyopathy, as these are adverse effects that have been reported [2]

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Briefly, plasma samples, as well as standard and control samples, were added (5 L/well) to a 96-well microplate pre-coated with a monoclonal antibody specific for insulin