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Cells were washed twice and analysis were performed using a FACSVerse flow cytometer (BD Biosciences, US), and the FlowJo (Version 10) software

In summary, HUVECs (labeled with lenti-mCherry) and HBVPCs (labeled with lenti-EGFP, and transfected with siCtrl or si PLXNB1 ) were trypsinized and suspended in a mixture containing 5 mg/mL fibrinogen (Sigma, #F8630) with 0.15 U/mL aprotinin (Sigma, #A1153-25 MG) at a density of 8 10 6 cells/mL for HUVECs and 8 10 5 cells/mL for HBVPCs

On the other hand, a single carton extends larger than a cigarette package

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