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Then cells were diluted with 0.51 ml assay buffer, and 10,000 events were analysed by flow cytometry using a BD Accuri C6 Plus flow cytometer (BD Biosciences) and FCS Express 7 Research (DeNovo Software)

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For this analysis, we used three online resources: (1) NetCorona (cbs.dtu.dk/services/NetCorona) (12), the only resource specific for predictions of M pro cleavage sites in coronaviruses (although not limited to just the SARS type coronaviruses), (2) PROSPER, the Protease Specificity Prediction Server (prosper.erc.monash.edu.au) (11), which was rated in a recent study as one of the most accurate resources for prediction of HIV-1 protease sites (20), and (3) Procleave (cited above), which was only used if either of the first 2 methods failed to identify a site

Using a USP-grade solvent ensures that your lyophilized compounds do not degrade prematurely through oxidation or hydrolysis

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