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After 48 h, blood samples were collected via retro-orbital puncture and analyzed at the clinical chemistry laboratory of the University Hospital Dresden

DSIP (Delta Sleep-Inducing Peptide) is a 9-amino-acid linear nonapeptide isolated in 1977 from the cerebral venous blood of rabbits during electrically induced sleep

Needle reuse can cause dulling and barb formation, significantly increasing injection pain

Bacteriostatic water has become the standard in most research settings because it maintains sterility through repeated vial access, preserves peptide integrity without interference, and supports efficient multi-dose protocols

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