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Cell fraction was centrifuged again at 10,000 rpm for 10 minutes at 4 C, and PBS was removed

The residue was dissolved in a 2 mL volumetric flask using DMSO- d 6 + 0.05% tetramethylsilane (99.9% D, Cambridge Isotope Labs) NMR solvent containing 26 L of HPLC-grade H 2 O (Honeywell) and an NMR quantification standard, 2,3,4,5-tetrachloronitrobenzene (TCI Chemicals, 902 M)

To make things even easier, the nicotine salt e-liquid in each disposable vaporizer is already inside

And I will say it again they are really very different