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EXAMPLE 13: CONFIRMATION ON THE EFFECT OF TONICITY AGENT TYPE IN THE HISTIDINE-BUFFERED FORMULATION AND THE EFFECT OF THE CITRATE- BUFFERED FORMULATION ON THE SUBCUTANEOUS TOLERANCE UPON SUBCUTANEOUS INJECTION This experiment examines: (1) the effect that the type of tonicity agent has on the local tolerance profile, upon subcutaneous injection, of an otherwise identical, histidine-buffered co-formulation

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After washes with PBS, cells were fixed with 1% PFA in PBS and inspected under under a Zeiss Axioplan fluorescence microscope

Intracellular GSH levels are controlled by Nrf2 expression (30)

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