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Bioorg Med Chem Lett 22:18221826 Eaton JK, Furst L, Ruberto RA, Moosmayer D, Hilpmann A, Ryan MJ, Zimmermann K, Cai LL, Niehues M, Badock V, Kramm A, Chen S, Hillig RC, Clemons PA, Gradl S, Montagnon C, Lazarski KE, Christian S, Bajrami B et al (2020) Selective covalent targeting of GPX4 using masked nitrile-oxide electrophiles

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Signs a peptide needs acetic acid water instead of BAC: the solution is cloudy or milky, you see a gel-like consistency, visible particles remain after 30+ minutes, or the powder fails to dissolve no matter how long you wait

Next, we run the solution through a 0.22-micron filter

Hughes, K