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Total RNA was extracted from the cell lysates using an EZ1 RNA Cell Mini Kit (Qiagen GmbH) on EZ1 Advanced XL automated RNA purification instrument (Qiagen GmbH) following the manufacturer's instruction

After first puncture: the 28-day window The storage scenario changes entirely at first puncture

Adult is diluted in an intravenous infusion, refrigerate the resulting solution unless it is to be used immediately, and use the solution within 24 hours after dilution

Epithalon peptide, known for its potential anti-aging properties, operates at the cellular level by influencing the function of telomeres, which are protective caps at the ends of chromosomes

Clinical oversight required: Dosage adjustments, reconstitution, and injection technique should always be guided by a licensed provider