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The resulting sperm samples were washed twice with 1X PBS, centrifuged (4000 g for 3 min), and flash frozen in liquid nitrogen before storage at 80 C for ensuing analyses

The choice to switch to e-cigarettes must be made easier, she said.

"Chronic exposure to tobacco smoke damages the airways and lung parenchyma, leading to the development of chronic obstructive pulmonary disease (COPD) characterised by airflow limitation and respiratory symptoms," Dr Amuthan states

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