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The PCR product was subjected to restriction digestion with 10 U of the Bss T1I enzyme (Sibenzyme) at 60 C for 4 h followed by 2.5%-agarose gel electrophoresis

Several studies have shown that accumulating AGEs and advanced oxidation protein products (AOPPs) in humans leads to tissue destruction and dysfunction 74

10.1096/fj.08-128926 28 KlaimanC.HuffmanL.MasakiL.ElliottG

There are several causes of vitamin B12 deficiency, including poor dietary intake, malabsorption, and pernicious anemia

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