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Proteins were transferred to PVDF membranes and blocked with 5% non-fat milk at room temperature for 1 h

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A key extrinsic pathway component is the death receptor Fas (CD95), which, upon binding to Fas ligand (FasL/CD95L), recruits the adaptor protein FADD (Fas-associated death domain protein) and procaspase-8 to form the death-inducing signaling complex (DISC)