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By the 1920s, cigarette smoking emerged as the dominant form of tobacco consumption

Cells were washed with cold PBS and lysed in a buffer containing 10 mmol/L Tris-HCl (pH 7.4), 50 mmol/L NaCl, 5 mmol/L ethylene diamine tetra-acetic acid (EDTA), 1% Nonidet P-40

Funding This work was supported by the intramural funding received from IISER Bhopal, Madhya Pradesh, India

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