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The plate were then incubated at 37 C, with 5% of CO 2 and 95% of humidity during 45 min, after which 50 L of reaction mixture was added to each well and incubated for additional 30 min at room temperature in the dark

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Annatto-extracted tocotrienols improve glucose homeostasis and bone properties in high-fat diet-induced type 2 diabetic mice by decreasing the inflammatory response

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