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Libraries were sequenced on a HiSeq 4000 device (Illumina, San Diego, CA) using a HiSeq 3000/4000 PE Cluster Kit and two 50-cycle SBS kits (Illumina, San Diego, CA), reaching an average mean depth of 61,000 (16% CV) and 50,000 (5% CV) mapped reads per cell per sample for the acute and sub-chronic models, respectively

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Full Chapter | Parallel Translations | Explain Verse John 16:33 KJV 33 These things I have spoken unto you, that in me ye might have peace

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The dense and properly humidified tobacco leaves, the comforting and smooth taste of rightly made rolling paper give you the sense that this is how smoking was meant to be