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Once the cells reached 5060% confluence, the medium was replaced with 1 mL of medium containing 5 g/mL polybrene (Genechem), along with either lentivirus-containing shRNA targeting Lsm2 (shLsm2) or lentivirus vectors (negative control, NC)

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Although many of these additives are used in making cigarettes, each cigarette does not contain all of these additives