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Exposure to CSE from 3R4F, NF and ultralow nicotine cigarettes negatively impacts ZO-1 and occludin expression/distribution as well as BBB integrity Immunofluorescence analysis of BBB endothelial confluent monolayers revealed a significant loss of ZO-1 at cell-cell junctions following exposure to CSE from 3R4F, Ultralow and NF cigarettes and to a lesser extent in 1R5F treated cultures (compared to controls, Figure 4A)