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Histology, immunohistochemistry and immunofluorescence staining for lung tissues Formalin-fixed and paraffin-embedded lung tissues of mice were cut in sections, which were stained with hematoxylin and eosin (HE), Periodic acidSchiff (PAS) and MASSON to respectively evaluate airway inflammation, goblet cell hyperplasia and mucous secretion, and extracellular matrix in the lung tissues [26]
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