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All samples (approximately 200 g each) were subjected to reduction with 20 mM tris (2-carboxyethyl) phosphine (TCEP) in 100 mM triethylammonium bicarbonate buffer (TEAB) and alkylated by 55 mM chloroacetamide (CAA) at room temperature for 30 min in the dark

The 15-amino acid sequence (Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val) demonstrates remarkable stability in gastric juice conditions and has been extensively investigated in regenerative research models for over 30 years

Common tirzepatide side effects include nausea, reduced appetite, and mild gastrointestinal symptomsnone of which worsen or improve based on whether you inject before or after meals

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