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Seedling tissue homogenates (20 g per lane) were run on SDSPAGE gel and western blot analysis was conducted following an established procedure ( ROS Production Measurement Wild type, atgrxs17 KO, and AtGRXS17 RNAi seeds were germinated and grown on MS medium for 5 days, then transferred and grown on iron sufficient or deficient medium for 3 days before seedlings were collected for measurement of ROS production in roots