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Research on hookah smoking found that one hookah session delivers approximately 125 times the smoke, 25 times the tar, 2.5 times the nicotine and 10 times the carbon monoxide compared with a single cigarette

Subcellular fractionation Nuclear, mitochondrial and cytosolic fractions were separated using the Mitochondria Isolation Kit for Cultured Cells (89874, ThermoFisher Scientific) according to the manufacturers specifications

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