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In general, direct damage refers to the direct injury to DNA induced by ionizing radiation

Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

The level of OGD-induced ferroptosis in ADSCs was assessed according to the viability of cells treated with the ferroptosis inhibitor Ferrostatin-1 (Fer-1) 5 M, autophagy inhibitor 3-Methyladenine (3-MA) 5 mM, apoptosis inhibitor Z-VAD-FMK 10 M, necroptosis inhibitor Necrostatin-1 (Nec-1) 50 M, or ferroptosis activator erastin

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