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Flavonoids were quantified using peak areas of A 280nm , A 500nm or selective ion monitoring in positive mode with external calibration curves prepared in the same solvent as the samples

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According to the US National Academy of Medicine, plasma -tocopherol concentrations less than 12 mol/L (516 g/dL) in adults are indicative of vitamin E inadequacy (2)

2.4 Cell proliferation assay The effect of formononetin on cell proliferation ability was determined by CCK8 assay and plate clone formation assay