CircLigase-ssDNA Ligase, 1,000 U Chemicals RNAs with a 5´-diphosphorylated end

RNAs with a 5´-diphosphorylated end are also converted to 5´-monophosphorylated RNA by RNA 5´ Polyphosphatase:

The protocol is extremely flexible and easily adjusted if there are varying volumes to be purified

biocompatibility and sterility

prior their being quality tested for optimal and reproducible impedance measurements

The BuccalFix Plus DNA Isolation Kits are designed to easily and quickly purify DNA already stored in BuccalFix Stabilization buffers

CircLigase-ssDNA Ligase, 1,000 U Chemicals RNAs with a 5´-diphosphorylated endThermostable ligase that catalyzes the intramolecular ligation (i. e. circularization) of ssDNA templates Intramolecular ligation of single stranded DNA ends containing 5' phosphates and 3' hydroxyl groups ATP dependent, catalytic enzyme Circularizes ssDNA >30 bases long Compatible with high temperature ligation reactions due to thermostability of CircLigase enzyme Applications Production of ssDNA templates for rolling circle replication or rolling