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One µl of Ready-Lyse Solution was added to each suspension and the cells were incubated at room temperature for 30 minutes
Transformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction
PCR product from cDNA synthesized by Epicentre's MMLV HP RT
(1971) Proc
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MU-GLU size:100mg One µl of Ready-Lyse Solution
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