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Environments acting during early embryogenesis (e.g., when global erasure and re-establishment of DNA methylation occur) may induce extensive, soma-wide modifications of DNA methylation, whereas environments acting later during life are more likely to induce less extensive, tissue-specific modifications of DNA methylation ( Figure 1 )

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Therefore, SHS induces both oxidative DNA damage and repair, as well as inflammation as possible underlying mechanism(s) of the behavioral and cognitive function and metabolic changes that were observed in chronically exposed mice (Raber et al., 2021)

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Randomization Participants will be randomized to 1 of 3 study conditions modeling different regulatory scenarios using stratified block randomization