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Then, 90 l of MSTFA with 1% TMCS derivatization agent (#TS-48915, Thermo Fisher Scientific, Pierce Biotechnology, Rockford, IL) was added in concomitance with 2 l of a mixture of C8-C30 FAMEs (# 400505, Fiehn GC/MS Metabolomics Standards Kit, Agilent Technologies, Santa Clara, CA, USA), used as markers of retention times and incubated at 37 C for 30 min under shaking conditions

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The chromatogram was recorded via UV absorption at 210 nm

However, you must consult your doctor to use glutathione for skin lightening