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it does not actively kill all microbes (that would be bactericidal), but it stops them from reproducing, which is sufficient to maintain sterility in a closed multi-dose vial

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Load the suspension on to a solution of 18% (w/v) Nycodenz containing 0.13 M NaCl, 3 mM KCl, 0.3 mM CaNa2EDTA, 5 mM Tris-HCl, pH 7.2 and centrifuged at 35,000 g for 40 min (or 27,000 g for 1 h) in a swinging-bucket rotor for an ultracentrifuge or high-speed centrifuge respectively

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Therapeutic strategies that restore redox balance, normalize iron handling, and preserve mitochondrial integrity may therefore target a central mechanism in EMs pathogenesis