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Mice were group-housed on 12 h:12 h light cycle with ambient temperature of 2026 C and relative humidity in range of 4070%

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Lipid- and protein-rich tissues often require additional pre-treatment steps (e.g., fat removal, detergent/ethanol washing, alkaline saponification), which are necessary for matrix reduction but may alter particle surfaces, promote aggregation, or partially degrade polymers [6, 171]