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PLoS One 7 , e42088 (2012)

Subsequently, all samples were subjected to centrifugation at 1500 rpm for 15 min at 4 C, facilitating a clear delineation of the two phases: the upper (lipophilic) phase, which contains the apolar metabolites, and the lower (hydrophilic) phase, which comprises the polar metabolites of interest

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Cholesterol levels are a significant risk factor for atherosclerosis, as demonstrated by a study by Habas and Shang (106), which found that copper deficiency elevated cholesterol levels and impacted lipid metabolism thereby influencing the development of atherosclerosis (107)