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To avoid feeling too energized close to bedtime, which can affect your sleep, you should stick to taking your B vitamins in the morning, preferably with a meal, to enhance their absorption

Prog Neurobiol 62:649671 Dringen R, Hirrlinger J (2003) Glutathione pathways in the brain

Yilmaz et al, 2011

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For vWF assessment, the cells were fixed with 4% paraformaldehyde, washed three times with PBS and incubated in 10% bovine serum albumin for 30 min to block non-specific binding