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Quantitative and digital PCR Quantitative PCR was performed on purified genomic DNA using TaqMan Advanced Master Mix (Applied Biosystems), 250 nM primers and 333 nM probes (sequences listed in Supplementary Dataset 1), and a QuantStudio6 (Applied Biosystems)

When v3 integrin activation is blocked, SHP-2 is instead recruited to IGF-1R where it dephosphorylates and so terminates the activation of IGF-1R (Zheng and Clemmons, 1998

Angiogenesis research: Studies have documented the ability of this compound to stimulate angiogenic signalling in laboratory models, particularly through interactions with VEGF and FGF pathway components, making it relevant to wound biology and vascular research

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